Optimisation of RNA extraction from Gracilaria changii (Gracilariales, Rhodophyta)
نویسندگان
چکیده
منابع مشابه
Total RNA Extraction for the Red Seaweed Gracilaria changii ( Gracilariales , Rhodophyta )
Five different RNA extraction methods have been tried out on the red seaweed, Gracilaria changii collected from the mangrove area at Morib, Selangor, Malaysia. Two methods, one utilising guanidinium thiocyanate, and another using cetyltrimethyl ammonium bromide (CTAB), were found to be potential alternatives to obtain pure RNA. By incorporating sand while grinding the tissue, the method using C...
متن کاملAnti-inflammatory, gastroprotective and anti-ulcerogenic effects of red algae Gracilaria changii (Gracilariales, Rhodophyta) extract
BACKGROUND Gracilaria changii (Xia et Abbott) Abbott, Zhang et Xia, a red algae commonly found in the coastal areas of Malaysia is traditionally used for foods and for the treatment of various ailments including inflammation and gastric ailments. The aim of the study was to investigate anti-inflammatory, gastroprotective and anti-ulcerogenic activities of a mass spectrometry standardized methan...
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Seaweeds survive in marine waters with high sulfate concentration compared to those living at freshwater habitats. The cell wall polymer of Gracilaria spp. which supplies more than 50% of the world agar is heavily sulfated. Since sulfation reduces the agar quality, it is interesting to investigate the effects of sulfate deprivation on the sulfate contents of seaweed and agar, as well as the met...
متن کاملIdentification of phase relative genes in tetrasporophytes and female gametophytes of Gracilaria/Gracilariopsis lemaneiformis (Gracilariales, Rhodophyta)
* Corresponding author Genes differentially expressed between tetrasporophytes and female gametophytes of Gracilaria/Gracilariopsis lemaneiformis were isolated by suppression subtractive hybridization (SSH) and screened by dot-blot macroarrays. Different expression profiles of selected clones based on the results of dot-blot macro-arrays were verified using virtual Northern blots. Totally, 14 p...
متن کاملRoutine DNA barcoding of Canadian Gracilariales (Rhodophyta) reveals the invasive species Gracilaria vermiculophylla in British Columbia.
As part of an extensive DNA-based floristic survey of marine macroalgae in Canadian waters, an unexpected sequence for a Gracilaria sp. was generated from British Columbia. Before further molecular analyses and corresponding morphological/anatomical observations this mystery sequence was temporarily entered into our database as Gracilaria BCsp. Continued sampling uncovered this species from fou...
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ژورنال
عنوان ژورنال: Journal of Applied Phycology
سال: 2004
ISSN: 0921-8971
DOI: 10.1023/b:japh.0000047782.20940.de